Journal: Cell metabolism

Article Title: Global analysis of plasma lipids identifies liver-derived acylcarnitines as a fuel source for brown fat thermogenesis

doi: 10.1016/j.cmet.2017.08.006

Figure Lengend Snippet: A) Heat Map and cluster analysis of 281 lipids from plasma of 3 month-old C57BL6J male mice at room temperature (24°C) or Cold (4°C). Samples were run on an Agilent 6490 LC-MS and analyzed using Metaboanalyst 3.0 (N = 5/group). B) Volcano plot of LC-MS based lipidomics from the plasma of 3 month-old C57BL6J male mice at Room Temp or Cold. Lipids that are increased 2-fold in Cold/RT and have a p-value below 0.01 are labeled in red. Long chain acylcarnitine species were identified through LC-MS/MS (N = 5/group). C) SAM analysis of LC-MS lipidomics of plasma from room temperature vs cold exposed in C57BL6J male mice 3 month or 24 month-old (N=4–5/group). D) Plasma acylcarnitine levels of 3 month and 24 month-old C57BL6J male mice at room temperature Room Temp or Cold (N=4–5/group). ◆ Data are presented as mean ± SEM. *p ≤ 0.05, ***p ≤ 0.001, room temp. vs cold. p ≤ 0.05 3 months vs 24 months Room Temp. E) Plasma levels of ◆ long chain acylcarnitine (LCAC), medium chain acylcarnitines (MCAC), short chain acylcarnitines (SCAC), and carnitine in the plasma of 3 month and 24 month-old C57BL6J male mice as measured by LC-MS (N=4–5/group). Data are presented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, room temp. vs cold. p ≤ 0.05 3 months vs 24 months Room Temp. See also Figure S1.

Article Snippet: Acylcarnitines were quantified using stable isotope dilution method and NeoLynx software.

Techniques: Clinical Proteomics, Liquid Chromatography with Mass Spectroscopy, Labeling

Journal: Molecular Metabolism

Article Title: Mitochondrial disease disrupts hepatic allostasis and lowers the threshold for immune-mediated liver toxicity

doi: 10.1016/j.molmet.2020.100981

Figure Lengend Snippet: Hepatic steatosis in LivCox10 −/− livers after influenza infection. (A) Representative EMs of livers isolated from uninfected mice (left column) or after PR8 infection (right column). Black arrows indicate mitochondria. White arrows indicate lipid. N: nucleus. Scale bar = 1 μm. N = 4 mice/group. Data are representative of three experiments. (B) Representative ORO stains of liver tissue prepared from uninfected (right column) or PR8-infected mice. Scale bar = 50 μm. N = 4 mice/group. Data are representative of three experiments. (C) Expression of PPARα protein in lysates prepared from liver tissue harvested from uninfected (solid circles) or PR8-infected (open circles) LivCox10 +/+ and LivCox10 −/− mice. N = 3–4 mice/group. (D) Expression of ACADVL (top) and HADHA (bottom) proteins in liver lysates. N = 3–4 mice/group. (E) Heatmap of z-scores from acylcarnitine analyses of livers. N = 8 mice/group. Data represent mean ± SEM. ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001.

Article Snippet: For acylcarnitine analysis, liquid chromatography tandem mass spectrometry was performed on frozen liver samples homogenized in PBS by the Genetics Laboratory of the Kennedy Krieger Institute of Johns Hopkins Medical Center ( https://www.kennedykrieger.org/patient-care/centers-and-programs/genetics-laboratories ).

Techniques: Infection, Isolation, Expressing

Journal: Diabetologia

Article Title: A sustained increase in plasma NEFA upregulates the Toll-like receptor network in human muscle

doi: 10.1007/s00125-013-3111-x

Figure Lengend Snippet: Effect of lipid infusion on total myocellular concentrations of ceramide and DAG (a) (n = 12) and of ceramide species (b), DAG species (c) (n = 7) and acylcarnitine species (d) (n=10). Results are means ± SE. *p < 0.05 and **p < 0.01 vs saline infusion

Article Snippet: Acylcarnitine content Muscle acylcarnitine content was measured by Lipomics Services, Metabolon (West Sacramento, CA, USA).

Techniques: Saline